Seminar

High Throughput Screening of Fluorogenic RNA Aptamers Using Droplet-based Microfluidics for Live Cellular RNA Imaging

Speaker

Miss Dey Poonam Aditi

MPhil candidate in the Mechanical Engineering Dept.

Date & Time

Monday, 25 April 2022

2:30 am

Venue

Via Zoom

Abstract:

Live cellular RNA imaging has become an indispensable tool in cellular biology as vital roles of biologically significant RNAs are discovered. Fluorogenic RNA aptamers are single-stranded oligonucleotides that exhibit strong fluorescence upon target-binding and showcase great potential in real-time RNA visualization. Therefore, we have developed a droplet-based microfluidic platform that combines the generation, injection, and sorting of droplets, for high-throughput screening of fluorogenic RNA aptamers. The sorting is programmed to select sequences that specifically bind to a new green fluorescent protein (GFP) fluorophore-mimic from a large DNA library by generating millions of droplets, each containing no more than one DNA sequence, and sorting the top 0.5%-2% of the fluorescent droplets at about 175 Hz. We have completed three rounds of sequence enrichment with our microfluidic platform, with droplets exhibiting strong fluorescence following each selection round.

Research Areas:

Advanced Materials and Biomedical Engineering

Contact for

Information

Prof. A. Shum

+(852) 3917 7904

Download Details

in PDF